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05a  (Cell Applications Inc)


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    Cell Applications Inc 05a
    05a, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+preadipocytes%3A+hpad/bio_rxiv__64898__2025__12__18__695158-135-9-4?v=Cell+Applications+Inc
    Average 94 stars, based on 31 article reviews
    05a - by Bioz Stars, 2026-08
    94/100 stars

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    Cellular cultivation of human subcutaneous preadipocytes and differentiation into mature adipocytes in monolayer under 2D (top row) and 3D (bottom row) conditions. (A) Plating in monolayer; (B) differentiation; (C) Oil Red O staining.

    Journal: Endocrine Connections

    Article Title: Irisin attenuates SARS-CoV-2 entry into cells and cell damage in 2D and 3D cultures of human subcutaneous adipocytes

    doi: 10.1530/EC-25-0046

    Figure Lengend Snippet: Cellular cultivation of human subcutaneous preadipocytes and differentiation into mature adipocytes in monolayer under 2D (top row) and 3D (bottom row) conditions. (A) Plating in monolayer; (B) differentiation; (C) Oil Red O staining.

    Article Snippet: Human subcutaneous preadipocytes (Catalog #: HPAd-802s-05A) were obtained from Cell Applications, Inc. (USA) through Sigma-Aldrich (USA).

    Techniques: Staining

    SARS-CoV-2 pseudovirus capture levels, observed by fluorescence levels read in a microplate reader at excitation of 506 nm and emission of 517 nm (Green Reporter assay), in 2D and 3D cell cultures of human subcutaneous preadipocytes (C: Control group, cells with no treatment; ACE2: cells with ACE2 expression optimized; ACE2+PV: ACE2 cells with ACE2 expression optimized and exposed to SARS-CoV-2 pseudovirus (PV); and ACE2+I+PV: ACE2 cells with ACE2 expression optimized, treated with 20 nM irisin for 24 h, and exposed to SARS-CoV-2 pseudovirus (PV)). All groups were analyzed in triplicated samples and tested with ANOVA, followed by Tukey’s test. ** P < 0.01; n.s., not significant.

    Journal: Endocrine Connections

    Article Title: Irisin attenuates SARS-CoV-2 entry into cells and cell damage in 2D and 3D cultures of human subcutaneous adipocytes

    doi: 10.1530/EC-25-0046

    Figure Lengend Snippet: SARS-CoV-2 pseudovirus capture levels, observed by fluorescence levels read in a microplate reader at excitation of 506 nm and emission of 517 nm (Green Reporter assay), in 2D and 3D cell cultures of human subcutaneous preadipocytes (C: Control group, cells with no treatment; ACE2: cells with ACE2 expression optimized; ACE2+PV: ACE2 cells with ACE2 expression optimized and exposed to SARS-CoV-2 pseudovirus (PV); and ACE2+I+PV: ACE2 cells with ACE2 expression optimized, treated with 20 nM irisin for 24 h, and exposed to SARS-CoV-2 pseudovirus (PV)). All groups were analyzed in triplicated samples and tested with ANOVA, followed by Tukey’s test. ** P < 0.01; n.s., not significant.

    Article Snippet: Human subcutaneous preadipocytes (Catalog #: HPAd-802s-05A) were obtained from Cell Applications, Inc. (USA) through Sigma-Aldrich (USA).

    Techniques: Fluorescence, Reporter Assay, Control, Expressing